Amoebiasis: Laboratory diagnosis relied on isolation of cyst in the stool sample.

Cysts of $Entamoeba$: Sensitive and cannot survive by boiling.

Protozoa: Multiply in host in several hours.

MATS Hint: Replication time varies, but many protozoa have rapid multiplication.

Malaria: Fatal in all ages (non-immune) but severe in children <5.

Largest Intestinal Round Worm: $Ascaris$ $lumbricoides$.

Scotch Tape Test: Done to diagnose pinworm infection.

MATS Hint: $Enterobius$ $vermicularis$; eggs are laid perianally, detected by tape test.

Hot Air Oven Sterilization Temperature: $160^\circ C$.

Innate Host Defense: 1st Line of defense.

Differential Culture Medium: MacConkey agar.

Endotoxin: Toxic portion of molecule is lipid A.

Flagellar Basal Body of Gram Negative Bacteria: Possesses four rings.

MATS Hint: Gram-negative: 2 in plasma membrane, 2 in peptidoglycan and outer membrane.

Characteristic Shape of Bacteria: Maintained by peptidoglycans.

Eukaryotes: Usually has introns.

Bacterial Structure for Antibiotic Action: Cell wall.

Staining Tissue with Best Carmine: Suggests glycogen.

Formaldehyde: Is used 37% aqueous solution.

$Clostridium$ $tetani$: Produces neurotoxin.

MATS Hint: Tetanospasmin toxin causes spastic paralysis by blocking inhibitory neurotransmitters.

$Streptococci$ beta hemolytic group A: Produces damage heart valves.

MATS Hint: $S$. $pyogenes$ can cause Rheumatic Heart Disease via molecular mimicry.

Triple Sugar Iron (TSI) Media: Slant alkaline but acidic, gas and blackening in media organism will be $Shigella$.

$Bacteria$ $fragilis$: Can produce intra abdominal abscesses.